Application
Immunocytochemistry of SIGIRR in A549 cells with SIGIRR antibody at 10 μg/mL.Application
Western blot analysis of SIGIRR in A549 cell lysate with SIGIRR antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | SIGIRR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIGIRR Antibody: SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily. Members of this family are defined by the presence of an intracellular Toll-IL-1R (TIR) domain. The Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SIGIRR was originally identified through database analysis and was shown to have only one Ig domain as opposed to the normal three Ig folds seen in the TIR family. Similar to ST2, another TIR family member, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor signaling. However, SIGIRR inhibits TLR-IL-1R signaling by dimerizing with TLR4, TLR5, TLR9, and IL-1R. It also associates with the down-stream TLR signaling proteins IRAK and TRAF6 in an IL-1-dependent fashion.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. |
| Immunogen | SIGIRR antibody was raised against a synthetic peptide corresponding to 15 amino acids at the C-terminus of human SIGIRR. The immunogen is located within the last 50 amino acids of SIGIRR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| SIGIRR antibody can be used for the detection of SIGIRR by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIGIRR |
|---|---|
| Alternative Names | SIGIRR Antibody: TIR8 Single Ig IL-1-related receptor Single Ig IL-1R-related molecule TIR8 |
| Sequence Similarities | 2) Cat. No. 17-003 - A549 Cell Slide |
Application
Immunocytochemistry of SIGIRR in A549 cells with SIGIRR antibody at 10 μg/mL.
Application
Western blot analysis of SIGIRR in A549 cell lysate with SIGIRR antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | SIGIRR antibody can be used for the detection of SIGIRR by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIGIRR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIGIRR Antibody is supplied in PBS containing 0.02% sodium azide. |
