Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | SILV Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SILV plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.1) Leonhardt, R.M., et al. J. Biol. Chem. 285(21):16166-16183(2010) |
| Immunogen | This SILV antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 412-440 amino acids from the Central region of human SILV. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PMEL |
|---|---|
| Alternative Names | Melanocyte protein PMEL ME20-M ME20M Melanocyte protein Pmel 17 Melanocytes lineage-specific antigen GP100 Melanoma-associated ME20 antigen P1 P100 Premelanosome protein Silver locus protein homolog M-alpha 95 kDa melanocyte-specific secreted gl |
| Molecular Weight(MW) | 70 kDa |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
