WB
Western blot analysis of SILV/PMEL using anti-SILV/PMEL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A2058 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SILV/PMEL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
SILV/PMEL was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was incubated with rabbit anti-SILV/PMEL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
SILV/PMEL was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was incubated with rabbit anti-SILV/PMEL Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Overlay histogram showing U2OS cells stained with anti-SILV/PMEL antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SILV/PMEL Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to SILV/PMEL |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Melanoma gp100/PMEL, which shares 71.4% and 65.7% amino acid (aa) sequence identity with mouse and rat Melanoma gp100/PMEL, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PMEL |
|---|---|
| Gene Synonyms | P1 SI SIL ME20 P100 SILV HMB45 ME20M gp100 HMB-45 ME20-M PMEL17 D12S53E |
| Gene Full Name | premelanosome protein |
| Gene Summary | This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011] |
| Alternative Names | D12S53E gp100 ME20 ME20 M ME20 S ME20M ME20S M beta Melanocyte protein Pmel 17 P1 P100 P26 PMEL PMEL17 SI SIL SILV Silver locus protein homolog |
| Molecular Weight(MW) | 70 kDa(Observed band size:80 kDa) |
| Cellular Localization | Endoplasmic reticulum,Endosome,Golgi apparatus,Membrane,Secreted |
WB
Western blot analysis of SILV/PMEL using anti-SILV/PMEL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A2058 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SILV/PMEL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
SILV/PMEL was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was incubated with rabbit anti-SILV/PMEL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
SILV/PMEL was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was incubated with rabbit anti-SILV/PMEL Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Overlay histogram showing U2OS cells stained with anti-SILV/PMEL antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SILV/PMEL Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
