Application
Immunocytochemistry of SIP1 in HeLa cells with SIP1 antibody at 4 μg/mL.Application
Immunofluorescence of SIP1 in HeLa cells with SIP1 antibody at 20 μg/mL.Application
Western blot analysis of SIP1 in HeLa cell lysate with SIP1 antibody at (A) 0.5 and (B) 1μg/mL.| Product Name | SIP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIP1 Antibody: SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.1) Liu Q, Fischer U, Wang F, et al. The spinal muscle atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell1997; 90:1013-21. |
| Immunogen | SIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human SIP1. The immunogen is located within the last 50 amino acids of SIP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SIP1 antibody can be used for detection of SIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIP1 |
|---|---|
| Alternative Names | SIP1 Antibody: SIP1 SIP1-delta SIP1 Gem-associated protein 2 Component of gems 2 Gemin-2 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of SIP1 in HeLa cells with SIP1 antibody at 4 μg/mL.
Application
Immunofluorescence of SIP1 in HeLa cells with SIP1 antibody at 20 μg/mL.
Application
Western blot analysis of SIP1 in HeLa cell lysate with SIP1 antibody at (A) 0.5 and (B) 1μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | SIP1 antibody can be used for detection of SIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
