WB
Various lysates were subjected to SDS PAGE followed by western blot with ZEB2 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using ZEB2 antibody at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using ZEB2 antibody at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ZEB2 antibody at dilution of 1:500 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | ZEB2 Recombinant antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ZEB2 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:2000-1:10000 IHC:1:600-1:2400 ICC/IF:1:250-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZEB2 |
|---|---|
| Gene Synonyms | SIP1 SIP-1 ZFHX1B HSPC082 SMADIP1 |
| Gene Full Name | zinc finger E-box binding homeobox 2 |
| Gene Summary | The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010] |
| Alternative Names | SIP1 1K22 HRIHFB2411 KIAA0569 SIP 1 |
| Molecular Weight(MW) | 140-150 kDa |
| Cellular Localization | Chromosome,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ZEB2 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using ZEB2 antibody at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using ZEB2 antibody at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ZEB2 antibody at dilution of 1:500 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:2000-1:10000 IHC:1:600-1:2400 ICC/IF:1:250-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
