Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Intestine.| Product Name | SKIIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SKIIP (Nuclear Protein SkiP, nuclear receptor coactivator NCoA-62, ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly (A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.1) Figueroa, J.D., et al., (2004) Eur.Res.Commun.319(4), 1105-1109. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SKIIP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SKIIP antibody can be used for detection of SKIIP by ELISA at 1:62500. SKIIP antibody can be used for detection of SKIIP by western blot at 1.0-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNW1 |
|---|---|
| Alternative Names | SKIIP Bx42 SKIP Prp45 SKIIP PRPF45 NCOA-62 |
| Molecular Weight(MW) | 61 kDa |
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Intestine.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SKIIP antibody can be used for detection of SKIIP by ELISA at 1:62500. SKIIP antibody can be used for detection of SKIIP by western blot at 1.0-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SKIIP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
