Application
Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.Application
Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.| Product Name | SkiP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SkiP Antibody: Bx42, SKIP, Prp45, SKIIP, PRPF45, NCOA-62, SNW domain-containing protein 1, Nuclear protein SkiP |
| Product description | SkiP Antibody: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.1) Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129. |
| Immunogen | SkiP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human SkiP. The immunogen is located within the last 50 amino acids of SkiP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SkiP antibody can be used for detection of SkiP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNW1 |
|---|---|
| Alternative Names | SkiP Antibody: Bx42 SKIP Prp45 SKIIP PRPF45 NCOA-62 SNW domain-containing protein 1 Nuclear protein SkiP |
| Sequence Similarities | 3) Cat. No. 10-401 - Human Kidney Tissue Slide |
Application
Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
Application
Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.| Positive Control | 1) Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | SkiP antibody can be used for detection of SkiP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SkiP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SkiP Antibody is supplied in PBS containing 0.02% sodium azide. |
