Application
Immunohistochemistry of Smac/DIABLO in human ovary tissue with Smac antibody at 5 μg/mL.Application
Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2409P) with Smac antibody at 1 μg/mL.| Product Name | Smac Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Smac Antibody: Smac, AU040403, 0610041G12Rik, 1700006L01Rik, Smac, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI |
| Product description | Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.1) Du C, Fang M, Li Y, et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 2000; 102:33-42. |
| Immunogen | Smac antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Smac. The immunogen is located within the last 50 amino acids of Smac. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Smac antibody can be used for detection of Smac/DIABLO by Western blot at 1 μg/mL. Smac antibody can also be used to detect Smac/DIABLO by immunohistochemistry at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Diablo |
|---|---|
| Alternative Names | Smac Antibody: Smac AU040403 0610041G12Rik 1700006L01Rik Smac Diablo homolog mitochondrial Direct IAP-binding protein with low pI |
| Molecular Weight(MW) | Predicted: 26 kDa Observed: 25 kDa |
| Sequence Similarities | 3) Cat. No. 11-201 - Human Ovary Tissue Slide |
Application
Immunohistochemistry of Smac/DIABLO in human ovary tissue with Smac antibody at 5 μg/mL.
Application
Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2409P) with Smac antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | Smac antibody can be used for detection of Smac/DIABLO by Western blot at 1 μg/mL. Smac antibody can also be used to detect Smac/DIABLO by immunohistochemistry at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Smac antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Smac Antibody is supplied in PBS containing 0.02% sodium azide. |
