Application
Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 μg/mL.Application
Immunofluorescence of Smac in Mouse Spleen cells with Smac antibody at 10 μg/mL.Application
Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 μg /ml.| Product Name | Smac Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Smac Antibody: Smac, AU040403, 0610041G12Rik, 1700006L01Rik, Smac, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI |
| Product description | Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.1) Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell. 2000;102(1):33-42. |
| Immunogen | Smac antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine Smac. The immunogen is located within the last 50 amino acids of Smac. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PIPWB |
| Smac antibody can be used for detection of Smac by Western blot at 1 μg/mL. A 25 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Diablo |
|---|---|
| Alternative Names | Smac Antibody: Smac AU040403 0610041G12Rik 1700006L01Rik Smac Diablo homolog mitochondrial Direct IAP-binding protein with low pI |
| Molecular Weight(MW) | 25 kDa |
Application
Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 μg/mL.
Application
Immunofluorescence of Smac in Mouse Spleen cells with Smac antibody at 10 μg/mL.
Application
Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 μg /ml.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | Smac antibody can be used for detection of Smac by Western blot at 1 μg/mL. A 25 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Smac antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Smac Antibody is supplied in PBS containing 0.02% sodium azide. |
