Application
Antibody used in WB on r-SMAD5 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug ofApplication
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | SMAD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.1) Zimonjic, D.B., et al., (2003) Neoplasia 5 (5), 390-396. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD5 |
|---|---|
| Alternative Names | SMAD5 DWFC JV5-1 MADH5 |
| Molecular Weight(MW) | 51 kDa |
Application
Antibody used in WB on r-SMAD5 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug of
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
