Application
Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.Application
Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 μg/ml.Application
Immunohistochemistry of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 2 μg/ml.Application
Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.Application
Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 μg/ml.| Product Name | SMARCA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1). Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2). The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3). In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4,5).1) Muchardt C and Yaniv M. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor. EMBO J. 1993; 12:4279-90. |
| Immunogen | SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4. The immunogen is located within amino acids 1420 - 1470 of SMARCA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMARCA4 |
|---|---|
| Alternative Names | SMARCA4 Antibody: BRG1 SNF2 SWI2 MRD16 RTPS2 BAF190 SNF2L4 SNF2LB hSNF2b BAF190A BRG1 SNF2B Transcription activator BRG1 ATP-dependent helicase SMARCA4 |
| Molecular Weight(MW) | Predicted: 185 kDa Observed: 200 kDa |
| Entrez Gene | 6597 |
|---|---|
| Protein Accession | NP_001122321 |
Application
Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.
Application
Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 μg/ml.
Application
Immunohistochemistry of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 2 μg/ml.
Application
Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.
Application
Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SMARCA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide. |
