WB
Various lysates were subjected to SDS PAGE followed by western blot with SMARCA4/BRG1 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).IP
IP result of anti-SMARCA4/BRG1 with HeLa cells lysate 3200 ug.| Product Name | SMARCA4/BRG1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Excitation/Emission maxima wavelengths: 588 nm / 604 nm |
| Immunogen | Recombinant human SMARCA4 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC/IF:1:400-1:1600 WB:1:5000-1:50000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMARCA4 |
|---|---|
| Gene Synonyms | BRG1 CSS4 SNF2 SWI2 MRD16 RTPS2 BAF190 OTSC12 SNF2L4 SNF2LB hSNF2b BAF190A SNF2-beta |
| Gene Full Name | SWI/SNF related BAF chromatin remodeling complex subunit ATPase 4 |
| Gene Summary | The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| Alternative Names | SMARCA4 2E6B6 ATP dependent helicase SMARCA4 ATP-dependent helicase SMARCA4 BAF190 |
| Molecular Weight(MW) | 185 kDa |
| Cellular Localization | Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SMARCA4/BRG1 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
IP
IP result of anti-SMARCA4/BRG1 with HeLa cells lysate 3200 ug.| Application Notes | ICC/IF:1:400-1:1600 WB:1:5000-1:50000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
