Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.Application
Antibody used in WB on Human, Mouse, Rat 1:500 (Lane 1: HeLa (human)Lane 2: NHEM (human)Lane 3: Melba (mouse)Lane 4: NIH3T3 (mouse)Lane 5: S16 (rat)Lane 6: H9C2 (rat) ).Application
Antibody used in IHC on Human kidney.| Product Name | SMARCE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMARCE1 is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.1) Chen, J. (2005) Mol. Cell. Biol. 25 (20), 9016-9027. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMARCE1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| SMARCE1 antibody can be used for detection of SMARCE1 by ELISA at 1:12500. SMARCE1 antibody can be used for detection of SMARCE1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMARCE1 |
|---|---|
| Alternative Names | SMARCE1 BAF57 |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Application
Antibody used in WB on Human, Mouse, Rat 1:500 (Lane 1: HeLa (human)Lane 2: NHEM (human)Lane 3: Melba (mouse)Lane 4: NIH3T3 (mouse)Lane 5: S16 (rat)Lane 6: H9C2 (rat) ).
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SMARCE1 antibody can be used for detection of SMARCE1 by ELISA at 1:12500. SMARCE1 antibody can be used for detection of SMARCE1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMARCE1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
