Application
Western blot analysis of SMURF1 in 293 cell lysate with SMURF1 antibody at 1 μg/ml.Application
Immunohistochemistry of SMURF1 in rat kidney tissue with SMURF1 antibody at 5 μg/ml.| Product Name | SMURF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).1) Huibregtse JM, Scheffner M, Beaudenon S, et al. A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 1995; 92:2563-7. |
| Immunogen | SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1. The immunogen is located within amino acids 180 - 230 of SMURF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMURF1 |
|---|---|
| Alternative Names | SMAD specific E3 ubiquitin protein ligase 1 SMAD ubiquitination regulatory factor 1 E3 ubiquitin-protein ligase SMURF1 |
| Molecular Weight(MW) | Predicted: 80, 83 kDa Observed: 78 kDa |
Application
Western blot analysis of SMURF1 in 293 cell lysate with SMURF1 antibody at 1 μg/ml.
Application
Immunohistochemistry of SMURF1 in rat kidney tissue with SMURF1 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SMURF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SMURF1 antibody is supplied in PBS containing 0.02% sodium azide. |
