WB
Western blot analysis of Smurf1 expression in HeLa (A) and Hep G2 (B) w.hole cell lysateWB
Western blot analysis of Smurf1 expression in MDA-MB-231 whole cell lysate.IHC
Immunoperoxidase staining of human gastric tissue fixed in formalin and embedded in paraffin reveals cytoplasmic localization.ICC/IF
Immunofluorescence staining of HeLa cells fixed with paraformaldehyde reveals cytoplasmic localization.| Product Name | Mouse Monoclonal antibody to Smurf1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against a partial recombinant peptide (165-269) Smurf1 of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Protein A |
| Gene Symbol | SMURF1 |
|---|---|
| Gene Full Name | SMAD specific E3 ubiquitin protein ligase 1 |
| Gene Summary | This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010] |
| Alternative Names | SMURF1 E3 ubiquitin ligase SMURF1 KIAA1625 HSMURF1 |
| Molecular Weight(MW) | 87kDa |
| Cellular Localization | Cytoplasm, Cell membrane. |
WB
Western blot analysis of Smurf1 expression in HeLa (A) and Hep G2 (B) w.hole cell lysate
WB
Western blot analysis of Smurf1 expression in MDA-MB-231 whole cell lysate.
IHC
Immunoperoxidase staining of human gastric tissue fixed in formalin and embedded in paraffin reveals cytoplasmic localization.
ICC/IF
Immunofluorescence staining of HeLa cells fixed with paraformaldehyde reveals cytoplasmic localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 100 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
