Application
Immunohistochemical analysis of paraffin - embedded human glioma tissue showing membrane location using SNCA antibody with DAB staining.Application
Western blot analysis using SNCA monoclonal antibody against truncated SNCA recombinant protein.| Product Name | SNCA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Alpha-synuclein (SNCA), with 140-amino acid protein (about 15 kDa), belongs to the synuclein family, which also includes beta- and gamma-synuclein. SNCA is a soluble protein, expressed principally in the brain but also expressed in low concentrations in all tissues examined (except liver). SNCA is implicated in the regulation of dopamine release and transport. The triplication of the SNCA can cause Parkinson disease (PD) and diffuse Lewy body disease within the same kindred. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Immunohistochemistry for SNCA has become the histological technique of choice for the diagnosis for Parkinson's disease, Dementia with Lewy bodies and Multiple System Atrophy.1) J. Johnson, S. M. Hague, M. Hanson. Neurology, Aug 2004; 63: 554 – 556. |
| Immunogen | Ni-NTA purified truncated recombinant SNCA expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000 Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SNCA |
|---|---|
| Alternative Names | NACP PARK1 Non-A beta component of AD amyloid NACP PD1 PARK4 |
Application
Immunohistochemical analysis of paraffin - embedded human glioma tissue showing membrane location using SNCA antibody with DAB staining.
Application
Western blot analysis using SNCA monoclonal antibody against truncated SNCA recombinant protein.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000 Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SNCA monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
