Application
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Product Name | SOCS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SOCS1 Antibody: The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines. SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase. SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome. It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP.1) Rakesh K and Agrawal DK. Controlling cytokine signaling by constitutive inhibitors. Biochem. Pharm. 2005; 70:649-57. |
| Immunogen | SOCS1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human SOCS1. The immunogen is located within the first 50 amino acids of SOCS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| SOCS1 antibody can be used for the detection of SOCS1 by Western blot at 1 - 4 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOCS1 |
|---|---|
| Alternative Names | SOCS1 Antibody: JAB CIS1 SSI1 TIP3 CISH1 SSI-1 SOCS-1 Suppressor of cytokine signaling 1 JAK-binding protein |
Application
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | SOCS1 antibody can be used for the detection of SOCS1 by Western blot at 1 - 4 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SOCS1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SOCS1 Antibody is supplied in PBS containing 0.02% sodium azide. |
