Immunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: SOCS1Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
SOCS1 antibody - N-terminal region validated by WB using Huh7 Cells transfected with microRNA at 1:1000.Western blot
1.25ug/mlSample Type: Jurkat Lysate (20ug)Â Sample Type: Jurkat cell lysateWestern blot
SOCS1 antibody - N-terminal region validated by WB using Jurkat cell lysate at 0.2-1 ug/ml.| Product Name | Rabbit anti-SOCS1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3 |
| Immunogen | The immunogen for anti-SOCS1 antibody: synthetic peptide directed towards the N terminal of human SOCS1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SOCS1 |
|---|---|
| Gene Synonyms | CIS1 CISH1 JAB SOCS-1 SSI-1 SSI1 TIP3 |
| Gene Full Name | Suppressor of cytokine signaling 1 |
| Gene Summary | SOCS1 is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. SOCS1 functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of its gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival.This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. |
| Alternative Names | CIS1 CISH1 JAB SOCS-1 SSI-1 SSI1 TIP3 |
| Molecular Weight(MW) | 23kDa |
| Sequence | 211 amino acids |
Immunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: SOCS1Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
SOCS1 antibody - N-terminal region validated by WB using Huh7 Cells transfected with microRNA at 1:1000.
Western blot
1.25ug/mlSample Type: Jurkat Lysate (20ug)Â Sample Type: Jurkat cell lysate
Western blot
SOCS1 antibody - N-terminal region validated by WB using Jurkat cell lysate at 0.2-1 ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
