WB
Western Blot analysis of KB cell lysate using SOCS-1 Polyclonal Antibody diluted at 1:2000.IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,SOCS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of mouse-liver tissue. 1,SOCS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B| Product Name | SOCS1 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SOCS1 Antibody: The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines. SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase. SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome. It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP.1) Rakesh K and Agrawal DK. Controlling cytokine signaling by constitutive inhibitors. Biochem. Pharm. 2005; 70:649-57. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SOCS-1. AA range:49-98 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:300 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOCS1 |
|---|---|
| Alternative Names | SOCS1 Antibody: JAB CIS1 SSI1 TIP3 CISH1 SSI-1 SOCS-1 Suppressor of cytokine signaling 1 JAK-binding protein |
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | Nucleus,Cytoplasmic vesicle. |
| Entrez Gene | 8651 |
|---|
WB
Western Blot analysis of KB cell lysate using SOCS-1 Polyclonal Antibody diluted at 1:2000.
IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,SOCS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of mouse-liver tissue. 1,SOCS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B| Application Notes | WB:1:500-1:2000 IHC:1:50-1:300 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
