Application
Immunocytochemistry of SODD in HeLa cells with SODD antibody at 5 μg/mL.Application
Immunofluorescence of SODD in Hela cells with SODD antibody at 20 μg/mL.Application
Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution.| Product Name | SODD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SODD Antibody: SODD, BAG-4, SODD, BAG family molecular chaperone regulator 4, Bcl-2-associated athanogene 4 |
| Product description | SODD Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.1) Jiang Y, Woronicz JD, Liu W, Goeddel DY. Prevention of constitutive TNF receptor 1 signaling by silencer of death domains. Science 1999;283:543-6 (RD1299) |
| Immunogen | SODD antibody was raised against a peptide corresponding to amino acids near the amino terminus of human SODD. The immunogen is located within the first 50 amino acids of SODD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SODD antibody can be used for detection of SODD by Western blot at 0.5 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAG4 |
|---|---|
| Alternative Names | SODD Antibody: SODD BAG-4 SODD BAG family molecular chaperone regulator 4 Bcl-2-associated athanogene 4 |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of SODD in HeLa cells with SODD antibody at 5 μg/mL.
Application
Immunofluorescence of SODD in Hela cells with SODD antibody at 20 μg/mL.
Application
Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | SODD antibody can be used for detection of SODD by Western blot at 0.5 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SODD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SODD Antibody is supplied in PBS containing 0.02% sodium azide. |
