Application
Immunohistochemical analysis of paraffin - embedded human normal cerebrum (top and bottom) showing cytoplasma location using SORL1 antibody with DAB staining.Application
Western blot analysis using SORL1 monoclonal antibody against truncated SORL1 - MBP recombinant protein.| Product Name | SORL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C11orf32, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LDLR relative with 11 ligand-binding repeats, LR11, LRP9, SORLA, gp250, SorLA-1, C11orf32 |
| Product description | SORL1 (sortilin-related receptor, L A repeats containing) also known as sorting protein-related receptor containing LDLR class A (SorLA), is a Type I membrane protein that may be involved in cell-cell interaction. SorLA, a single transmembrane receptor, binds LDL and transports it into cells by endocytosis. SorLA is synthesized as a proreceptor which is processed to the mature form by a furin-like propeptidase. It can also bind to RAP (receptor-associated protein). SorLA is a multifunctional endocytis receptor important in lipoprotein and protease uptake. The N-terminal propeptide, which is removed, can be cleaved by furin or homologous proteases. Endogenous SorLA binds the neuropeptide head activator (HA) and is important for HA signaling and function. The gene encoding for the protein maps to chromosome 8p23.1. SorLA is expressed mainly in brain (cerebral cortex, cerebellum and the occipital pole), but can also be found in liver, spinal cord, kidney, testis and pancreas.1) Scherzer CR. Offe K. Gearing M. et al. Arch Neurol. 2004, Aug, 61(8):1200-5. |
| Immunogen | Purified truncated recombinant SORL1-MBP expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SORL1 |
|---|---|
| Alternative Names | C11orf32 Low-density lipoprotein receptor relative with 11 ligand-binding repeats LDLR relative with 11 ligand-binding repeats LR11 LRP9 SORLA gp250 SorLA-1 C11orf32 |
Application
Immunohistochemical analysis of paraffin - embedded human normal cerebrum (top and bottom) showing cytoplasma location using SORL1 antibody with DAB staining.
Application
Western blot analysis using SORL1 monoclonal antibody against truncated SORL1 - MBP recombinant protein.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SORL1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
