Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SOST antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of lysates from human kidney and liver tissue lysates (from left to right), using SOST Antibody at 1:1000 at each lane.| Product Name | SOST Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of the sclerostin gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.1) Semenov,M.V., J. Biol. Chem. 281 (50), 38276-38284 (2006) |
| Immunogen | This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-163 amino acids from the Central region of human SOST. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SOST |
|---|---|
| Alternative Names | Sclerostin SOST |
| Molecular Weight(MW) | 24 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SOST antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of lysates from human kidney and liver tissue lysates (from left to right), using SOST Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
