WB
Western blot analysis of lysates from Jurkat cells, primary antibody was diluted at 1:1000, 4°over night.IHC
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).| Product Name | Sclerostin Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from Sclerostin . at AA range: 130-210. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SOST |
|---|---|
| Gene Synonyms | CDD VBCH DAND6 SOST1 |
| Gene Full Name | sclerostin |
| Gene Summary | Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease. [provided by RefSeq, Jul 2008] |
| Alternative Names | SOST Sclerostin |
| Molecular Weight(MW) | 26kDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix . |
WB
Western blot analysis of lysates from Jurkat cells, primary antibody was diluted at 1:1000, 4°over night.
IHC
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
