Application
Immunocytochemistry of SPT2 in 3T3 cells with SPT2 antibody at 10 μg/mL.Application
Immunofluorescence of SPT2 in 3T3 cells with SPT2 antibody at 20 μg/mL.Application
Western blot analysis of SPT2 in 3T3 cell lysate with SPT2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SPT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPT2 Antibody: LCB2, SPT2, HSN1C, LCB2A, NSAN1C, hLCB2a, KIAA0526, LCB2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, LCB 2 |
| Product description | SPT2 Antibody: Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.1) Batheja AD, Uhlinger DJ, Carton JM, et al. Characterization of serine palmitoyltransferase in normal human tissues. J. Histochem. & Cytochem. 2003; 51:687-96. |
| Immunogen | SPT2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SPT2. The immunogen is located within the last 50 amino acids of SPT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SPT2 antibody can be used for detection of SPT2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPTLC2 |
|---|---|
| Alternative Names | SPT2 Antibody: LCB2 SPT2 HSN1C LCB2A NSAN1C hLCB2a KIAA0526 LCB2 Serine palmitoyltransferase 2 Long chain base biosynthesis protein 2 LCB 2 |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | At least three isoforms of SPT2 are known to exist; this antibody will not detect isoform b. SPT2 antibody is predicted to not cross-react with SPT1. |
Application
Immunocytochemistry of SPT2 in 3T3 cells with SPT2 antibody at 10 μg/mL.
Application
Immunofluorescence of SPT2 in 3T3 cells with SPT2 antibody at 20 μg/mL.
Application
Western blot analysis of SPT2 in 3T3 cell lysate with SPT2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | SPT2 antibody can be used for detection of SPT2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPT2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPT2 Antibody is supplied in PBS containing 0.02% sodium azide. |
