Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | SPTLC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPTLC2, KIAA0526, LCB2, SPT2, HSN1C, LCB2A, NSAN1C, hLCB2a |
| Product description | SPTLC2 is a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I.This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. Alternatively spliced variants encoding different isoforms have been identified.1) Hornemann, T., (2007) Biochem. J. 405 (1), 157-164. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPTLC2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SPTLC2 antibody can be used for detection of SPTLC2 by ELISA at 1:312500. SPTLC2 antibody can be used for detection of SPTLC2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPTLC2 |
|---|---|
| Alternative Names | SPTLC2 KIAA0526 LCB2 SPT2 HSN1C LCB2A NSAN1C hLCB2a |
| Molecular Weight(MW) | 63 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SPTLC2 antibody can be used for detection of SPTLC2 by ELISA at 1:312500. SPTLC2 antibody can be used for detection of SPTLC2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPTLC2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
