Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with SRC1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | SRC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclear receptor coactivator 1, NCoA-1, Class E basic helix-loop-helix protein 74, bHLHe74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, SRC-1, NCOA1, BHLHE74, SRC1 |
| Product description | SRC1 acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. This protein binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion.1) Lavery,D.N.,Biochemistry 47 (11), 3352-3359 (2008) |
| Immunogen | This SRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1338-1367 amino acids from the C-terminal region of human SRC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NCOA1 |
|---|---|
| Alternative Names | Nuclear receptor coactivator 1 NCoA-1 Class E basic helix-loop-helix protein 74 bHLHe74 Protein Hin-2 RIP160 Renal carcinoma antigen NY-REN-52 Steroid receptor coactivator 1 SRC-1 NCOA1 BHLHE74 SRC1 |
| Molecular Weight(MW) | 157 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with SRC1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
