Application
Immunocytochemistry of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL .Application
Immunofluorescence of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL.Application
Western blot analysis of SRSF3 in K562 cell lysate with SRSF3 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | SRSF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/arginine-rich splicing factor 3, Pre-mRNA-splicing factor SRP20, SFRS3, Splicing factor arginine/serine-rich 3, SRP20 |
| Product description | SRSF3, a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors which constitute part of the spliceosome, may be involved in RNA processing in relation with cellular proliferation and/or maturation (1,2). Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins (3). The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation (3,4).1) Sen S, Jumaa H, and Webster NJ. Splicing factor SRSF3 is crucial for hepatocyte differentiation and metabolic function. Nat. Commun. 2013; 4:1336. |
| Immunogen | SRSF3 antibody was raised against a 19 amino acid peptide near the center of human SRSF3. The immunogen is located within amino acids 80 - 130 of SRSF3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SRSF3 antibody can be used for detection of SRSF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 2 μg/mL.
For immunofluorescence start at 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRSF3 |
|---|---|
| Alternative Names | Serine/arginine-rich splicing factor 3 Pre-mRNA-splicing factor SRP20 SFRS3 Splicing factor arginine/serine-rich 3 SRP20 |
| Molecular Weight(MW) | Predicted: 18 kDa Observed: 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | SRSF3 antibody is human, mouse and rat reactive. |
Application
Immunocytochemistry of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL .
Application
Immunofluorescence of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL.
Application
Western blot analysis of SRSF3 in K562 cell lysate with SRSF3 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | SRSF3 antibody can be used for detection of SRSF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 2 μg/mL.
For immunofluorescence start at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SRSF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SRSF3 antibody is supplied in PBS containing 0.02% sodium azide. |
