Application
Immunohistochemistry of ST2 in human lung cancer tissue with ST2 antibody at 5 μg/ml.Application
Immunofluorescence of ST2 in human lung cancer tissue with ST2 antibody at 5 μg/ml.Application
Immunohistochemistry of ST2 in human liver tissue with ST2 antibody at 5 μg/mL.Application
Immunofluorescence of ST2 in Mouse Kidney cells with ST2 antibody at 20 μg/mL.Application
Immunohistochemistry of ST2 in mouse kidney tissue with ST2 antibody at 2 μg/mL.Application
Western blot analysis of ST2 in 293 cell lysate with ST2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | ST2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ST2 Antibody: T1, St2, DER4, Ly84, ST2L, Fit-1, T1/ST2, St2-rs1, Ste2, Interleukin-1 receptor-like 1, Lymphocyte antigen 84 |
| Product description | ST2 Antibody: ST2 is a member of a superfamily containing the interleukin-1 receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor (TLR) 4 signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. |
| Immunogen | ST2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino-terminus of mouse ST2. This peptide is common to all three known ST2 isoforms. The immunogen is located within amino acids 40 - 90 of ST2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ST2 antibody can be used for the detection of ST2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Il1rl1 |
|---|---|
| Alternative Names | ST2 Antibody: T1 St2 DER4 Ly84 ST2L Fit-1 T1/ST2 St2-rs1 Ste2 Interleukin-1 receptor-like 1 Lymphocyte antigen 84 |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of ST2 in human lung cancer tissue with ST2 antibody at 5 μg/ml.
Application
Immunofluorescence of ST2 in human lung cancer tissue with ST2 antibody at 5 μg/ml.
Application
Immunohistochemistry of ST2 in human liver tissue with ST2 antibody at 5 μg/mL.
Application
Immunofluorescence of ST2 in Mouse Kidney cells with ST2 antibody at 20 μg/mL.
Application
Immunohistochemistry of ST2 in mouse kidney tissue with ST2 antibody at 2 μg/mL.
Application
Western blot analysis of ST2 in 293 cell lysate with ST2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | ST2 antibody can be used for the detection of ST2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ST2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ST2 Antibody is supplied in PBS containing 0.02% sodium azide. |
