WB
Western blot analysis of ST2 on different lysates with Mouse anti-ST2 antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa overexpress with or without ST2 cells labeling ST2 with Mouse anti-ST2 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ST2 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HeLa overexpress with or without ST2 cells labeling ST2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a 594 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | ST2 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human ST2 aa 19-328 (Extracellular). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFWB |
| WB:1:1000 ICC/IF:1:100 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Il1rl1 |
|---|---|
| Gene Synonyms | T1 St2 DER4 Ly84 ST2L Fit-1 T1/ST2 St2-rs1 |
| Gene Full Name | interleukin 1 receptor-like 1 |
| Gene Summary | Enables interleukin-33 binding activity and interleukin-33 receptor activity. Involved in several processes, including macrophage differentiation; positive regulation of macrophage activation; and regulation of cytokine production. Acts upstream of or within negative regulation of canonical NF-kappaB signal transduction and response to wounding. Located in external side of plasma membrane and extracellular matrix. Is expressed in several structures, including alimentary system; immune system; musculoskeletal system; skin; and visual system. Human ortholog(s) of this gene implicated in acute asthma; allergic asthma; asthma; and coronary artery disease. Orthologous to human IL1RL1 (interleukin 1 receptor like 1). [provided by Alliance of Genome Resources, Jun 2026] |
| Alternative Names | DER-4 antibody DER4 antibody FIT 1 antibody Growth stimulation expressed antibody homolog of mouse growth stimulation-expressed antibody Il1rl1 antibody IL33R antibody ILRL1_HUMAN antibody Interleukin 1 receptor like 1 antibody interleukin 1 receptor related protein antibody Interleukin-1 receptor-like 1 antibody Protein ST2 antibody ST2 antibody ST2L antibody ST2V antibody T1 antibody T1 protein antibody |
| Molecular Weight(MW) | 63 kDa(Observed band size: 80 kDa) |
| Cellular Localization | Cell membrane, Membrane, Secreted. |
WB
Western blot analysis of ST2 on different lysates with Mouse anti-ST2 antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa overexpress with or without ST2 cells labeling ST2 with Mouse anti-ST2 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ST2 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HeLa overexpress with or without ST2 cells labeling ST2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a 594 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 ICC/IF:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
