Application
Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 μg/ml.Application
Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 μg/mL.| Product Name | STIM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STIM1 Antibody: GOK, TAM, IMD10, D11S4896E, GOK, Stromal interaction molecule 1 |
| Product description | STIM1 Antibody: In T lymphocytes, the sole pathway for Ca 2+ entry following antigen-receptor binding is through store-operated Ca 2+ -release-activated Ca 2+ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca 2+ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca 2+ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca 2+ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca 2+ entry.1) Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca 2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7. |
| Immunogen | STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1. The immunogen is located within the last 50 amino acids of STIM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STIM1 |
|---|---|
| Alternative Names | STIM1 Antibody: GOK TAM IMD10 D11S4896E GOK Stromal interaction molecule 1 |
| Molecular Weight(MW) | Predicted: 59, 75, 87 kDa Observed: 88 kDa |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2. |
Application
Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 μg/ml.
Application
Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1409 - Mouse Thymus Tissue Lysate |
|---|---|
| Application Notes | STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | STIM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | STIM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
