Application
Antibody used in WB on Human cell lines at: 1:2000 (Lane 1: 30ug of HeLa cell lysate, Lane 2: 30ug of 293T cell lysate).Application
Antibody used in WB on transfected Cos-7 at: 1:2000 (Lane1: 100ug untransfected COS-7 lysate, Lane2: 100ug mock transfected Cos-7 lysate, Lane3: 100ug STK3 transfected Cos-7 lysate, Lane4: 50 ug STK3 transfected Cos-7 lysate, Lane5: 25 ug STK3 transfected Cos-7 lysate).Application
Antibody used in WB on transfected Cos-7 at: 1:2000 (Lane1: 100ug untransfected RPE-1 lysate, Lane2: 100ug untransfected RPE-1 lysate, Lane3: 100ug STK3 induced RPE-1 lysate, Lane4: 100ug STK3 induced RPE-1 lysate).Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | STK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STK3, FLJ90748, KRS1, MST2 |
| Product description | Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand.Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-11 U26424.1 1-11 12-2826 BC010640.2 1-28151) Seidel, C., (2007) Mol. Carcinog. 46 (10), 865-871. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human STK3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| STK3 antibody can be used for detection of STK3 by ELISA at 1:312500. STK3 antibody can be used for detection of STK3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STK3 |
|---|---|
| Alternative Names | STK3 FLJ90748 KRS1 MST2 |
| Molecular Weight(MW) | 56 kDa |
Application
Antibody used in WB on Human cell lines at: 1:2000 (Lane 1: 30ug of HeLa cell lysate, Lane 2: 30ug of 293T cell lysate).
Application
Antibody used in WB on transfected Cos-7 at: 1:2000 (Lane1: 100ug untransfected COS-7 lysate, Lane2: 100ug mock transfected Cos-7 lysate, Lane3: 100ug STK3 transfected Cos-7 lysate, Lane4: 50 ug STK3 transfected Cos-7 lysate, Lane5: 25 ug STK3 transfected Cos-7 lysate).
Application
Antibody used in WB on transfected Cos-7 at: 1:2000 (Lane1: 100ug untransfected RPE-1 lysate, Lane2: 100ug untransfected RPE-1 lysate, Lane3: 100ug STK3 induced RPE-1 lysate, Lane4: 100ug STK3 induced RPE-1 lysate).
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | STK3 antibody can be used for detection of STK3 by ELISA at 1:312500. STK3 antibody can be used for detection of STK3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store STK3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
