SYVN1 is a protein involved in endoplasmic reticulum (ER)-associated degradation. The protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins.This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. This gene and the mitochondrial ribosomal protein L49 gene use in their respective 3' UTRs some of the same genomic sequence. Sequence analysis identified two transcript variants that encode different isoforms.1) Cattaneo, M., (2008) J. Cell. Physiol. 215 (3), 794-802.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SYVN1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SYVN1 antibody can be used for detection of SYVN1 by ELISA at 1:1562500. SYVN1 antibody can be used for detection of SYVN1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
SYVN1 antibody can be used for detection of SYVN1 by ELISA at 1:1562500. SYVN1 antibody can be used for detection of SYVN1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SYVN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.