Application
Western Blot at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: mouse spleen lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with SYVN1 (HRD1) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstainedApplication
Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 (1:1000) and anti-rabbit secondary POD-conjugated antibodies from Pierce Biotechnology, Inc (Rockford, IL, 1:2000)(Provided by Dr. Susana Granell & Institution University of Arkansas).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.| Product Name | SYVN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase synoviolin, 632-, Synovial apoptosis inhibitor 1, SYVN1, HRD1, KIAA1810 |
| Product description | HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Amano T, et al. reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects. HRD1 contains one ring-type zinc finger.1) References for protein: |
| Immunogen | This SYVN1 (HRD1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-617 amino acids from the C-terminal region of human SYVN1 (HRD1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:2000 For IF starting dilution is: 1:200 For IHC-P starting dilution is: 1:50~100 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | SYVN1 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase synoviolin 632- Synovial apoptosis inhibitor 1 SYVN1 HRD1 KIAA1810 |
| Molecular Weight(MW) | 68 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: mouse spleen lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with SYVN1 (HRD1) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained
Application
Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 (1:1000) and anti-rabbit secondary POD-conjugated antibodies from Pierce Biotechnology, Inc (Rockford, IL, 1:2000)(Provided by Dr. Susana Granell & Institution University of Arkansas).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.| Application Notes | For WB starting dilution is: 1:2000 For IF starting dilution is: 1:200 For IHC-P starting dilution is: 1:50~100 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
