Application
Western blot analysis of TAB1 in 3T3 cell lysate with TAB1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.Application
Immunofluorescence of TAB1 in 3T3 cells with TAB1 antibody at 2 μg/mL.Application
Immunocytochemistry of TAB1 in K562 cells with TAB1 antibody at 1 μg/mL.| Product Name | TAB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAB1 Antibody: 3'-Tab1, MAP3K7IP1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TAK1-binding protein 1 |
| Product description | TAB1 Antibody: TAB1 was identified as a regulator of the MAP kinase kinase kinase TAK1/MAP3K7, which is known to mediate various intracellular signaling pathways, such as those induced by TGF-beta and members of the Toll-IL-1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. This protein, together with either TAB2 or TAB3, activates TAK1 kinase in response to upstream signals. It has been shown that the C-terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF-beta, demonstrating how this protein can function as a mediator between TGF-beta receptors and TAK1.1) Shibuya H, Yamaguchi K, Shirakabe K, et al. TAB1: an activator of the TAK1 MAPKKK in TGF-β signal transduction. Science 1996; 272:1179-82. |
| Immunogen | TAB1 antibody was raised against a synthetic peptide corresponding to 13 amino acids in the center of human TAB1. The immunogen is located within amino acids 220 - 270 of TAB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TAB1 antibody can be used for the detection of TAB1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K7IP1 |
|---|---|
| Alternative Names | TAB1 Antibody: 3'-Tab1 MAP3K7IP1 TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 TAK1-binding protein 1 |
| Sequence Similarities | 4) Cat. No. 17-201 - 3T3/BALB Cell Slide |
Application
Western blot analysis of TAB1 in 3T3 cell lysate with TAB1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Application
Immunofluorescence of TAB1 in 3T3 cells with TAB1 antibody at 2 μg/mL.
Application
Immunocytochemistry of TAB1 in K562 cells with TAB1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | TAB1 antibody can be used for the detection of TAB1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TAB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TAB1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM292721