Application
Western Blot at 1:8000 dilution + H. uterus whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of TAGLN antibody in SK-Br-3 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of MDA-MB468 cells using TAGLN Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TAGLN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha, TAGLN, SM22, WS3-10 |
| Product description | TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.1) Zhao,L., Mod. Pathol. 22 (6), 786-796 (2009) Leguillette,R., Am. J. Respir. Crit. Care Med. 179 (3), 194-204 (2009) Yu,H., FASEB J. 22 (6), 1778-1789 (2008) |
| Immunogen | This TAGLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human TAGLN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TAGLN |
|---|---|
| Alternative Names | Transgelin 22 kDa actin-binding protein Protein WS3-10 Smooth muscle protein 22-alpha SM22-alpha TAGLN SM22 WS3-10 |
| Molecular Weight(MW) | 23 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:8000 dilution + H. uterus whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of TAGLN antibody in SK-Br-3 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of MDA-MB468 cells using TAGLN Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
