WB
Various lysates were subjected to SDS PAGE followed by western blot with transgelin/SM22 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin embedded human colon tissue slide using transgelin/SM22 antibody at dilution of 1:200 (under 10x lens).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody at dilution of 1:1000 and CoraLite®488 Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | transgelin/SM22 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TAGLN protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IF-PIHCWB |
| WB:1:5000-1:50000 IHC:1:50-1:400 IF-P:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAGLN |
|---|---|
| Gene Synonyms | SM22 SMCC TGLN TAGLN1 WS3-10 SM22-alpha |
| Gene Full Name | transgelin |
| Gene Summary | This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017] |
| Alternative Names | SM22 transgelin 22 kDa actin-binding protein 2A10C2 Protein WS3-10 |
| Molecular Weight(MW) | 22kDa |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with transgelin/SM22 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin embedded human colon tissue slide using transgelin/SM22 antibody at dilution of 1:200 (under 10x lens).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody at dilution of 1:1000 and CoraLite®488 Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:5000-1:50000 IHC:1:50-1:400 IF-P:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
