WB
HEK293T cells were transfected with the pCMV6 ENTRY control (Left lane) or pCMV6-ENTRY TAGLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAGLN.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min.ICC/IF
Anti-TAGLN mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TAGLN.| Product Name | SM22 alpha (TAGLN) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human reocombinant protein of human TAGLN(NP_003177) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:150 ICC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAGLN |
|---|---|
| Gene Synonyms | SM22 SMCC TGLN TAGLN1 WS3-10 SM22-alpha |
| Gene Full Name | transgelin |
| Gene Summary | This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017] |
| Alternative Names | SM22 SMCC TAGLN1 WS3-10 |
| Molecular Weight(MW) | 22.4kDa |
| Cellular Localization | Cytoplasm. |
WB
HEK293T cells were transfected with the pCMV6 ENTRY control (Left lane) or pCMV6-ENTRY TAGLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAGLN.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min.
ICC/IF
Anti-TAGLN mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TAGLN.| Application Notes | WB:1:500-1:2000 IHC:1:150 ICC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received,Stable for 12 months from date of receipt. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
