Application
Antibody used in WB on HepG2 at 1.25 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).| Product Name | TARDBP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TARDBP, ALS10, TDP-43 |
| Product description | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.1) Buratti, E., (2005) J. Biol. Chem. 280 (45), 37572-37584. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TARDBP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| TARDBP antibody can be used for detection of TARDBP by ELISA at 1:1562500. TARDBP antibody can be used for detection of TARDBP by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Dog |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TARDBP |
|---|---|
| Alternative Names | TARDBP ALS10 TDP-43 |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on HepG2 at 1.25 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TARDBP antibody can be used for detection of TARDBP by ELISA at 1:1562500. TARDBP antibody can be used for detection of TARDBP by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TARDBP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
