Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with TCHP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of TCHP Antibody in CEM cell line lysates (35ug/lane)Application
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TCHP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Trichoplein keratin filament-binding protein, Protein TCHP, Mitochondrial protein with oncostatic activity, Mitostatin, Tumor suppressor protein, TCHP {ECO:0000312|EMBL:AAH042851} |
| Product description | TCHP may act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. This protein may regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells.1) Vecchione,A. Oncogene 28 (2), 257-269 (2009) |
| Immunogen | This TCHP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-320 amino acids from the Central region of human TCHP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCHP |
|---|---|
| Alternative Names | Trichoplein keratin filament-binding protein Protein TCHP Mitochondrial protein with oncostatic activity Mitostatin Tumor suppressor protein TCHP {ECO:0000312|EMBL:AAH042851} |
| Molecular Weight(MW) | 61 kDa |
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with TCHP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of TCHP Antibody in CEM cell line lysates (35ug/lane)
Application
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
