Application
Flow cytometric analysis of Ramos cells using TCL1A Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.Application
Western blot analysis of lysate from Ramos cell line, using TCL1A Antibody at 1:1000.| Product Name | TCL1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1, Oncogene TCL1, Protein p14 TCL1, TCL1A, TCL1 |
| Product description | Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.1) Virgilio L.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:12530-12534(1994). |
| Immunogen | This TCL1A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-66 amino acids from the Central region of human TCL1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCL1A |
|---|---|
| Alternative Names | T-cell leukemia/lymphoma protein 1A Oncogene TCL-1 Oncogene TCL1 Protein p14 TCL1 TCL1A TCL1 |
| Molecular Weight(MW) | 13 kDa |
Application
Flow cytometric analysis of Ramos cells using TCL1A Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Application
Western blot analysis of lysate from Ramos cell line, using TCL1A Antibody at 1:1000.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
