Application
Western blot analysis using Trx monoclonal antibody against four different fusion protein with truncated Trx tag.| Product Name | Thioredoxin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLEC5B, NKRP1A, Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, NKR-P1A, NKR, CD161, NKR-P1, hNKR-P1A |
| Product description | Thioredoxin (TRX) is a small ubiquitous protein (MW12 kDa) which is exist in a wide variety of prokaryotic and eukaryotic cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site.This antibody is suitable for detecting fusion proteins which encode a Trx-Tag by immunoblotting and immunoprecipitation.The Monoclonal Antibody can detect a little Trx-Tag fusion proteins with negligible cross-reactivity with bacterial, insect, or mammalian lysates.1) Holmgen, A. et al., Annu. Rev. Biochem. 54, 237-271 (1985). |
| Immunogen | Recombinant human Thioredoxin (TRX). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Dilution Western Blot:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KLRB1 |
|---|---|
| Alternative Names | CLEC5B NKRP1A Killer cell lectin-like receptor subfamily B member 1 C-type lectin domain family 5 member B NKR-P1A NKR CD161 NKR-P1 hNKR-P1A |
Application
Western blot analysis using Trx monoclonal antibody against four different fusion protein with truncated Trx tag.| Application Notes | Dilution Western Blot:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Thioredoxin (TRX) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
