Application
Western blot analysis of extract from JK, T98G, COS7 and HeLa cells using TIF1β Antibody.| Product Name | TIF1beta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KAP1, TF1B, RNF96, TIF1B, KAP1 |
| Product description | Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.1) Friedman J. R., Fredericks W. J., Jensen D. E., Speicher D. W., Huang X. -P., Neilson E. G., Rauscher F. J. IIIGenes Dev. 10:2067-2078 (1996) Moosmann P. R., Georgiev O., le Douarin B., Bourquin J. -P., Schaffner W. Nucleic Acids Res. 24:4859-4867 (1996) Agata Y., Matsuda E., Shimizu A. J. Biol. Chem. 274:16412-16422 (1999) |
| Immunogen | TIF1beta antibody was raised against a peptide sequence around aa.827~831 (L-S-G-G-P) derived from Human TIF1β. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM28 |
|---|---|
| Alternative Names | KAP1 TF1B RNF96 TIF1B KAP1 |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | This antibody detects endogenous level of total TIF1β protein. |
| Entrez Gene | 10155 |
|---|---|
| Protein Accession | NP_005753.1 |
Application
Western blot analysis of extract from JK, T98G, COS7 and HeLa cells using TIF1β Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
