Application
Western blot analysis of TRIM28 in human testis tissue lysate with TRIM28 antibody at 1 μg/mL.| Product Name | TRIM28 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM28 Antibody: KAP1, TF1B, RNF96, TIF1B, KAP1, Transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, TIF1-beta |
| Product description | TRIM28 Antibody: TRIM28, also known as KAP-1, is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily and contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TRIM28 is a corepressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TRIM28 acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. TRIM28 associates with both heterochromatin and euchromatin, causing gene silencing by both HP1 binding and histone deacetylation.1) Friedman JR, Fredericks WJ, Jensen DE, et al. KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev. 1996; 10:2067-78. |
| Immunogen | Rabbit polyclonal TRIM28 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TRIM28. The immunogen is located within the last 50 amino acids of TRIM28. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| TRIM28 antibody can be used for detection of TRIM28 by Western blot at 1 - 2 μg/mL.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM28 |
|---|---|
| Alternative Names | TRIM28 Antibody: KAP1 TF1B RNF96 TIF1B KAP1 Transcription intermediary factor 1-beta E3 SUMO-protein ligase TRIM28 TIF1-beta |
| Molecular Weight(MW) | Predicted: 92 kDa |
| Tissue Specificity | At least three isoforms of TRIM28 are known to exist; this antibody will detect all three isoforms |
Application
Western blot analysis of TRIM28 in human testis tissue lysate with TRIM28 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | TRIM28 antibody can be used for detection of TRIM28 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIM28 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TRIM28 Antibody is supplied in PBS containing 0.02% sodium azide. |
