Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | TRIM28 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM28, KAP1, TF1B, RNF96, TIF1B |
| Product description | TRIM28 mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. TRIM28 localizes to the nucleus and is thought to associate with specific chromatin regions. It is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING,a B-box type 1 and a B-box type 2, and a coiled-coil region.1) Wang, C., et al., (2005) EMBO J. 24 (18), 3279-3290. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRIM28. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TRIM28 antibody can be used for detection of TRIM28 by ELISA at 1:312500. TRIM28 antibody can be used for detection of TRIM28 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TRIM28 |
|---|---|
| Alternative Names | TRIM28 KAP1 TF1B RNF96 TIF1B |
| Molecular Weight(MW) | 89 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Application Notes | TRIM28 antibody can be used for detection of TRIM28 by ELISA at 1:312500. TRIM28 antibody can be used for detection of TRIM28 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TRIM28 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
