Application
Antibody used in WB on Transfected 293T at 2.5 ug/ml.| Product Name | TIMELESS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TIMELESS, TIM, TIM1, hTIM |
| Product description | The human Timeless protein interacts with both the circadian clock protein cryptochrome 2 and with the cell cycle checkpoint proteins Chk1 and the ATR-ATRIP complex and plays an important role in the DNA damage checkpoint response. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein.1) Sangoram, A.M., (2005) Mol. Cell. Biol. 25 (8), 3109-3116. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TIMELESS. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TIMELESS antibody can be used for detection of TIMELESS by ELISA at 1:312500. TIMELESS antibody can be used for detection of TIMELESS by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TIMELESS |
|---|---|
| Alternative Names | TIMELESS TIM TIM1 hTIM |
| Molecular Weight(MW) | 139 kDa |
Application
Antibody used in WB on Transfected 293T at 2.5 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | TIMELESS antibody can be used for detection of TIMELESS by ELISA at 1:312500. TIMELESS antibody can be used for detection of TIMELESS by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TIMELESS antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
