Application
Western blot analysis of TIRAP in human heart tissue lysate with TIRAP antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TIRAP in Human Heart tissue with TIRAP antibody at 10 μg/mL.Application
Immunohistochemical staining of human heart tissue using TIRAP antibody at 2 μg/mL.| Product Name | TIRAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TIRAP Antibody: Mal, wyatt, BACTS1, MyD88-2, MAL, Adaptor protein Wyatt, TIR domain-containing adapter protein |
| Product description | TIRAP Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-kappaB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. |
| Immunogen | TIRAP antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of mouse TIRAP. The immunogen is located within the last 50 amino acids of TIRAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TIRAP antibody can be used for detection of TIRAP by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TIRAP |
|---|---|
| Alternative Names | TIRAP Antibody: Mal wyatt BACTS1 MyD88-2 MAL Adaptor protein Wyatt TIR domain-containing adapter protein |
| Molecular Weight(MW) | Predicted: 24, 26 kDa Observed: 24 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | At least three isoforms of TIRAP are know to exist; this antibody detects all three isoforms. |
Application
Western blot analysis of TIRAP in human heart tissue lysate with TIRAP antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TIRAP in Human Heart tissue with TIRAP antibody at 10 μg/mL.
Application
Immunohistochemical staining of human heart tissue using TIRAP antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | TIRAP antibody can be used for detection of TIRAP by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TIRAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TIRAP Antibody is supplied in PBS containing 0.02% sodium azide. |
