Application
Western blot analysis of TLR3 in EL4 cell lysate with TLR3 antibody at 0.5 μg/mL.Application
Immunofluorescence of TLR3 in K562 cells with TLR3 antibody at 2 μg/mL.Application
Immunocytochemistry of TLR3 in K562 cells with TLR3 antibody at 10 μg/mL.| Product Name | TLR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR3 Antibody: CD283, IIAE2, Toll-like receptor 3 |
| Product description | TLR3 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. |
| Immunogen | TLR3 antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human TLR3. The immunogen is located within amino acids 40 - 90 of TLR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| TLR3 antibody can be used for detection of TLR3 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR3 |
|---|---|
| Alternative Names | TLR3 Antibody: CD283 IIAE2 Toll-like receptor 3 |
| Molecular Weight(MW) | Predicted: 88 kDa Observed: 91 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Western blot analysis of TLR3 in EL4 cell lysate with TLR3 antibody at 0.5 μg/mL.
Application
Immunofluorescence of TLR3 in K562 cells with TLR3 antibody at 2 μg/mL.
Application
Immunocytochemistry of TLR3 in K562 cells with TLR3 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | TLR3 antibody can be used for detection of TLR3 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
