Application
Western blot analysis of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TLR3 in El4 cells with TLR3 antibody at 10 μg/mL.Application
Immunohistochemistry of TLR3 in human spleen tissue with TLR3 antibody at 5 μg/ml.Application
Immunocytochemistry of TLR3 in EL4 cells with TLR3 antibody at 1 μg/mL.| Product Name | TLR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR3 Antibody: CD283, IIAE2, Toll-like receptor 3 |
| Product description | TLR3 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. |
| Immunogen | TLR3 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR3. The immunogen is located within amino acids 780 - 830 of TLR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| TLR3 antibody can be used for detection of TLR3 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR3 |
|---|---|
| Alternative Names | TLR3 Antibody: CD283 IIAE2 Toll-like receptor 3 |
| Molecular Weight(MW) | 99 kDa |
| Sequence Similarities | 4) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Western blot analysis of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TLR3 in El4 cells with TLR3 antibody at 10 μg/mL.
Application
Immunohistochemistry of TLR3 in human spleen tissue with TLR3 antibody at 5 μg/ml.
Application
Immunocytochemistry of TLR3 in EL4 cells with TLR3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | TLR3 antibody can be used for detection of TLR3 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
