Application
Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 μg/mL.Application
Western blot analysis of TLR9 in mouse spleen cell lysate with TLR9 antibody at 1 μg/mL.Application
Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 5 μg/mL.| Product Name | TLR9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR9 Antibody: CD289, Toll-like receptor 9 |
| Product description | TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. |
| Immunogen | TLR9 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human TLR9. The immunogen is located within amino acids 960 - 1010 of TLR9. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:1000 IF:1:100~1:500 IHC-p:1:50~1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR9 |
|---|---|
| Alternative Names | TLR9 Antibody: CD289 Toll-like receptor 9 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (81%), Bovine: (100%), Sheep: (100%) |
Application
Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 μg/mL.
Application
Western blot analysis of TLR9 in mouse spleen cell lysate with TLR9 antibody at 1 μg/mL.
Application
Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:1000 IF:1:100~1:500 IHC-p:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR9 Antibody is supplied in PBS containing 0.02% sodium azide. |
