Application
Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptideApplication
Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 μg/mL.Application
Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 μg/mL.| Product Name | TLR9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR9 Antibody: CD289, Toll-like receptor 9 |
| Product description | TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76. |
| Immunogen | TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9. The immunogen is located within amino acids 840 - 890 of TLR9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TLR9 antibody can be used for detection of TLR9 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR9 |
|---|---|
| Alternative Names | TLR9 Antibody: CD289 Toll-like receptor 9 |
| Molecular Weight(MW) | Predicted: 104, 111 kDa Observed: 97 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins. |
Application
Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Application
Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 μg/mL.
Application
Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | TLR9 antibody can be used for detection of TLR9 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR9 Antibody is supplied in PBS containing 0.02% sodium azide. |
